Volume : VII, Issue : II, February - 2018

A comparative study of Ziehl Neelsen, Gabbet‘s and Fluorescent staining methods to detect acid fast bacilli in sputum samples.

Ms. Sharon Priya P. J, Dr. Thomas. S. Kuruvilla

Abstract :

 

              Tuberculosis  remains  worlds  leading  cause  of  death  from  a  single  infectious agent. Pulmonary  tuberculosis (PTB) is  the  most  common  presentation  of  tuberculosis. Here we compare Ziehl-Neelsen (ZN), Gabbets  and  Fluorescent staining (FS) methods  to  determine  efficiency  and cost effectiveness of diagnosing  pulmonary tuberculosis   from sputum  samples. Three  smear  from 148 sputum  samples  of  PTB cases were prepared and stained by the above methods. Of  the  samples  examined, 71.6% and 53.4% and 85.8% cases were detected  by  Ziehl-Neelsen,  Gabbets  and  Fluorescence  staining  respectively. Fluorescent staining  was  found to be more reliable and cost effective method  particularly  when  dealing with  large sample loads than Ziehl-Neelsen  and  Gabbets  staining technique.

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Article: Download PDF    DOI : https://www.doi.org/10.36106/gjra  

Cite This Article:

Ms. Sharon Priya P. J, Dr. Thomas. S. Kuruvilla, A comparative study of Ziehl Neelsen, Gabbet‘s and Fluorescent staining methods to detect acid fast bacilli in sputum samples., GLOBAL JOURNAL FOR RESEARCH ANALYSIS : VOLUME-7, ISSUE-2, FEBRUARY-2018


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