Volume : VII, Issue : IX, September - 2018

AN RP- HPLC METHOD DEVELOPMENT AND VALIDATION OF TRIAMTERENE AND BENZTHIAZIDE IN BULK AND PHARMACEUTICAL DOSAGE FORMS

M. Purushothaman, M. Rajasekaran, Rajesh Asija

Abstract :

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Triamterene and Benzthiazide, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol: Phosphate Buffer pH 3.5 (75:25) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 271 nm. The retention time of the Triamterene and Benzthiazidewas 2.344, 3.282 ±0.02min respectively. The method produce linear responses in the concentration range of 10-50mg/ml of Benzthiazide and 20-100mg/ml of Triamterene. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

Keywords :

Article: Download PDF    DOI : https://www.doi.org/10.36106/gjra  

Cite This Article:

M.Purushothaman, M.Rajasekaran, Rajesh Asija, AN RP- HPLC METHOD DEVELOPMENT AND VALIDATION OF TRIAMTERENE AND BENZTHIAZIDE IN BULK AND PHARMACEUTICAL DOSAGE FORMS, GLOBAL JOURNAL FOR RESEARCH ANALYSIS : Volume-7 | Issue-9 | September-2018


Number of Downloads : 264


References :