Volume : V, Issue : IV, April - 2016

Benzene biodegradation by a novel species of Bacillus megaterium

Manasa S, Jayachandran K

Abstract :

<p> In the present study, an attempt was made to evaluate the biodegradation profile of benzene by Bacillus megaterium. The bacterial culture was incubated at 6 intervals of time (8, 16, 24, 32, 40 and 48hr) in Mineral Salt Benzene Medium (MSBM). The intermediates were identified by TLC and HPTLC. Functional groups of the intermediates were confirmed by FT/IR analysis. Peak at 3300 cm-1 region of 8hr sample represented O-H group in aromatic ring of benzene. The spectrum represented new peaks in the range 1271 cm-1 -1039 cm-1 of 16hr indicating C-O bond, in 1726 cm-1 representing the C=O and in 900 cm-1 – 1000 cm-1 indicating shift in the C=C of aromatic ring. 24hr FT/IR gave evidence to suggest the presence of C-O bond (1100-1800 cm-1) and O-H (2880-3400 cm1). O-H stretching and C=C shift in the aromatic ring was further confirmed by the FT/IR at 32 hr incubation. FT/IR also helps in the analysis of the nature of the intermediates formed. The product of the degradation process was analyzed with GC/MS</p>

Keywords :

Article: Download PDF    DOI : https://www.doi.org/10.36106/gjra  

Cite This Article:

Manasa S, Jayachandran K Benzene biodegradation by a novel species of Bacillus megaterium Global Journal For Research Analysis, Vol.5, Issue : 4 APRIL 2016

Number of Downloads : 355

References :