CHANGES IN THE ABUNDANCE OF HEPATIC GENES INVOLVED IN SOME METABOLIC REGULATION AMONG DIFFERENT CATTLE GENOTYPE SLAUGHTERED AT BASRA SLAUGHTER HOUSE

Asaad Y. Ayied; Hadar H. Al-Bataat

Volume : VI, Issue : VII, July - 2017

CHANGES IN THE ABUNDANCE OF HEPATIC GENES INVOLVED IN SOME METABOLIC REGULATION AMONG DIFFERENT CATTLE GENOTYPE SLAUGHTERED AT BASRA SLAUGHTER HOUSE

Asaad Y. Ayied, Hadar H. Al Bataat

Abstract :

The current study was conducted at the laboratories of Agriculture College, University of Basra from 3/12/2015 till 13/10/2016. Liver samples were obtained from 100 cows divided into Brahman (24), cross (Friesian x Jenubi, 28), Jenubi (local eed, 26) and Roman (22) from Basra Slaughter House. Liver samples were analyzed for mRNA abundance of genes related to gluconeogenesis (mitochondrial phosphoenolpyruvate carboxykinase, PEPCKm), fatty acid oxidation (carnitine palmitoyl transferase 1A, CPT 1A), cholesterol biosynthesis (3-hydroxy-3-methylglutaryl-coenzyme A synthase 1, HMGCS1), ketogenesis (3-hydroxy-3-methylglutaryl-coenzyme A synthase 2, HMGCS2), triglyceride synthesis (Acetyl- CoA-Carboxylase, ACoC) and urea cycle related parameters (ornithine transcarbamylase, OCT) as well as housekeeping (reference) gene (Ribosomal protein S9, RPS9). The purpose of this study was to investigate the mRNA abundance variations of 6 different hepatic candidate genes responsible for some regulation of body metabolism among four different genotypes, sex and age. In addition, studying the differences in body live and carcass weight as well as dressing percentages among four studied genotypes, sex and age. The findings of the current study shows that the mRNA gene abundance related to cholesterol synthesis were observed to be significantly higher in all studied genotypes relative to Jenubi. Roman eed got two genes (OTC and CPT1A) with significantly down regulated mRNA gene abundance relative to Jenubi (-1.957 and -1.587 respectively). Brahman eed got one significant down regulated gene (CPT1A, -2.293). Females showed clear increased of mRNA abundance in almost all studied genes except OTC and CPT1A relative to male. The hepatic mRNA gene abundance of HMGCS1, PEPCKm, and CPT1A increased with animals‘ age, while the mRNA abundance of OTC, ACOC and HMGCS2 decreased relative to 2 years old.