DNA FINGER PRINTING OF SPECIES AND ACCESSIONS OF PLUMBAGO L. USING ISSR MARKERS

Prashant J. Gadge; Varsha N. Nathar

Volume : IV, Issue : VII, July - 2015

DNA FINGER PRINTING OF SPECIES AND ACCESSIONS OF PLUMBAGO L. USING ISSR MARKERS

Prashant J. Gadge, Varsha N. Nathar

Abstract :

Genetic diversity among species and accessions of Plumbago L. were estimated using ISSR marker. In this study the three species and two accessions were collected from different sites, DNA was isolated from each plant. Out of the 10 primers used 5 primers produced highly polymorphic DNA fragments. The total number of amplified DNA fragments was 126. Polymorphic Information Content (PIC), Resolving power (Rp) were also calculated. The PIC values for ISSR primers ranged from 0.768166 to 0.877915. Rp values calculated ranged from 6.8 to 11.6. The highest Rp value was observed in UBC-820 i.e. 11.6, whereas lowest in UBC-841 i.e. 6.8. Nei�s overall genetic diversity or heterozygosity was calculated and found 0.2194. The genetic identity between the population ranged from 0.51 to 0.86 and the genetic distance ranged from 0.14 to 0.49. The overall percentage of polymorphism was 55.00. Primer UBC 810 exhibit highest polymorphism i.e. 100%.