INDIRECT, DIRECT AND SECONDARY SOMATIC EMBRYOGENESIS IN EMBLICA OFFICINALIS

Volume : IV, Issue : IV, April - 2015

Priyanka, Upendra Kumar, K. P. Singh

A continuously growing callus from cotyledonary leaves was achieved in vitro on MS medium. After 2, 3 sub cultures 

on the same medium the callus became emyogenic and numerous emyos of different stages of development were 

observed on the surface of the callus. Direct somatic emyogenesis from cotyledons, and callus mediated (indirect) 

emyogenesis was observed when mature cotyledons were cultured on BMS medium supplemented with different concentrations ofauxins 

and cytokinins. 2,4–D alone, as well in combination of Kn led to somatic emyogenesis. BMS supplemented with IAA, IBA, BAP and Kn led to 

direct emyogenesis without callus formation. IAA and IBA on sub culture give rise to secondary emyos from culture raised emyos. When 

transferred on MS without hormone these emyos give rise to complete plants Plantlets were regenerated from the emyos 2 months after their 

transfer to the germination medium without any growth regulators but with the addition of 30 g/l sucrose. 