Influence of zona pellucida thickness of oocyte maturation and in vitro fertilization in the cow in Iraq

Saad Akram Hatif; Noor Al Mustafa Imad Kadhim

Volume : V, Issue : X, October - 2016

Influence of zona pellucida thickness of oocyte maturation and in vitro fertilization in the cow in Iraq

Saad Akram Hatif, Noor Al Mustafa Imad Kadhim

Abstract :

The aim of study to investigation the role of zona pellucida thickness in the oocytes maturation and in vitro fertilization in cows . This study included the collection of 200 female reproductive system from the abattoir .The ovaries collected and washing with the normal saline . Oocytes recovered via , aspiration and slicing, followed the maturation in TCM199 in Co2 incubator . Semen collected , diluents , and capacitation in order to In Vitro Fertilization . Under inverted microscopical examination the results revealed , oocytes classified in three types , complete surrounded by cumulus cells ,partial surrounded ,and denuded oocytes, synchronizing with deferent appearance of zona pellucida thickness , thin ,normal , and thick .The best thickness of zona pellucida in the oocyte ranged (15um- 17um) for successfully maturation and in vitro fertilization .