Linezolid � losing its golden touch to a golden bacteria

Dr. Dibyendu Banerjee; Dr. Baishali Chakraborty; Dr. Banya Chakraborty

Volume : V, Issue : IV, April - 2016

Linezolid � losing its golden touch to a golden bacteria

Dr. Dibyendu Banerjee, Dr. Baishali Chakraborty, Dr. Banya Chakraborty

Abstract :

<p>&nbsp;Background: Staphylococcus aureus is a very common and dangerous pathogen, causing pyogenic infections all over the world. This pathogen by becoming methicillin resistant (MRSA) became a cause of major concern to the medical fraternity. A chief alternative in the hand of clinicians is linezolid &ndash; an oxazolidinone. Materials and Methods: Cultures from100 wound swabs revealed 66 Staphylococcus aureus isolates. Among them, 4 Staphylococcus aureus showed resistance to Linezolid by disc diffusion method. They were found to be methicillin resistant as well. Then PCR of these 4 resistant strains as well as a sensitive strain was done followed by DNA sequencing. Results: Sequencing of the domain V region of the 23S rRNA gene revealed the presence of a G2576U mutation in the LRSA (LZD-resistant S. aureus) isolates in two 23s rRNA copy, rrn1 &amp; rrn5. Conclusions: We found a 6% incidence of LRSA, which were also MRSA, thus seriously jeopardizing treatment options. LRSA is a rare occurrence ,and rarely reported from Indian subcontinent. A constant vigil is necessary to detect this resistance ,if possible with tools of molecular epidemiology.</p>