Volume : IV, Issue : IV, April - 2015

Listeria monocytogenes biofilms on food contact surfaces under different growth conditions

K Vrinda Menon, B Sunil, C Latha, Nithya K. R, Ashok Kumar

Abstract :

<p> Listeria monocytogenes is an opportunistic intracellular pathogen that has become an important cause of foodborne </p> <div>infections worldwide. The organism possesses an ability to form biofilms on food processing surfaces, potentially </div> <div>leading to food product contamination. In the present study, stainless steel (SS) and cutting board fiber (FIB) coupons </div> <div>were used as the substratum for biofilm growth in the presence of sterile distilled water and Tryptone soya oth (TSB) as nutrient medium. The </div> <div>Listeria monocytogenes standard culture (MTCC 1143) belonging to serotype 4b at a concentration of 6 log 10 cfu/ ml was used for creating </div> <div>biofilms on these surfaces. The formation of biofilms was assessed after 24 hours of incubation at 370 C. The formation of biofilms on these </div> <div>surfaces after 24 hrs was observed by scanning electron microscopy (SEM) which revealed the presence of biofilms on both surfaces with more </div> <div>pronounced biofilm formation on fiber as compared to stainless steel in the presence of TSB as nutrient medium. The presence of irreversible </div> <div>biofilms with TSB as nutrient medium after washing the coupons was assessed which revealed the recovery of the organism at the level of 3.77 ± </div> <div>0.13 log 10 cfu/cm2 and 4.91± 0.33 log 10 cfu/cm2 on SS and FIB surfaces respectively. The presence of sterile distilled water as nutrient medium </div> <div>also revealed the recovery of the organisms but were at the level of 1.82 ± 0.18 log 10 cfu/cm2 and 2.80 ± 0.13 log 10 cfu/cm2 on SS and FIB </div> <div>surfaces respectively. The formation of biofilms even in the presence of sterile distilled water is an area of great concern for the food industry. </div> <div>However the study revealed that the difference in the characteristics of surfaces revealed a difference in the ability of the organism to form biofilm </div> <div>as the biofilm was more pronounced on the fiber as compared to stainless steel. Hence, biofilm growth of L. monocytogenes was sufficient to </div> <div>provide a substantial risk of this pathogen contaminating the food–processing plant environment if wet surfaces are not maintained in a sanitary </div> <div>condition.</div>

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Article: Download PDF    DOI : https://www.doi.org/10.36106/gjra  

Cite This Article:

K VRINDA MENON, B SUNIL, C LATHA, NITHYA K.R, ASHOK KUMAR Listeria monocytogenes biofilms on food contact surfaces under different growth conditions Global Journal For Research Analysis, Vol: 4, Issue: 4 April 2015

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