Phenotypic detection of carbapenemase production and molecular detection of blaOXA-51 and blaNDM-1 genes among the carbapenem resistant Enterobacteriaceae in a tertiary care rural teaching hospital

Dr.M.Jane Esther; Dr.Diego Edwin

Volume : VI, Issue : XI, November - 2017

Phenotypic detection of carbapenemase production and molecular detection of blaOXA-51 and blaNDM-1 genes among the carbapenem resistant Enterobacteriaceae in a tertiary care rural teaching hospital

Dr. M. Jane Esther, Dr. Diego Edwin

Abstract :

Introduction:

CRE contribute to increased morbidity and mortality among hospitalized patients. A knowledge of CRE prevalence helps in treatment and prevention of further spread of resistance.

Materials and methods:

All meropenem resistant Enterobacteriaceae isolates were subjected growth on KPC chrome agar, MHT, EDS test, CDT and genotyping for detection of baOXA-51 and blaNDM-1 genes.

Results and discussion:

Prevalence of CRE was 3.5%(25/713). 24/25(96%) CRE grew on KPC chrome agar, 64%(16/25) were positive for MHT, 80%(20/25) each for EDS and CDT. 13/25 harboured blaNDM-1, one blaOXA-51 and 7 both. We found good consistency between phenotypic and genotypic results. Ours is the pioneer study in India in detecting blaOXA-51 among Enterobacteriaceae.

Conclusion:

Even though the prevalence of CRE is low, appropriate infection control measures are needed to prevent further spread of resistance.