Studies on Designing, Optimisation and Application of Biosensor using Glucose Oxidase of Aspergillus niger F-C405-2

Ms. GOTHOSKAR VANDAN; Dr. Mrs. Smita M. Dharmadhikari; Dr. Mahendra D.Shirsat

Volume : IV, Issue : I, January - 2015

Studies on Designing, Optimisation and Application of Biosensor using Glucose Oxidase of Aspergillus niger F-C405-2

Ms. Gothoskar Vandan, Dr. Mrs. Smita M. Dharmadhikari, Dr. Mahendra D. Shirsat

Abstract :

Biosensor is a device which detects and combines with a biological component analysed with a physicochemical detector component. In present work we have designed a glucose biosensor using immobilised glucose oxidase enzyme on cellulose acetate produced by Aspergillus niger F– C405–2. This glucose oxidase (GO) has shown thermal stability at 600C for 120 minutes. Glucose oxidase was immobilized on pyrrol–cellulose acetate (Py–CA) film over Indium tin oxide (ITO). Platinum and silversilver chlorides are used as reference electrode and suitable for estimating blood sugar with commercial glucometer. The biosensor prepared was optimised and caliated against standard glucose solutions of variable concentration (0.5 to 6 mg/ml) with change in electric current measured in ampere. The amount of glucose present in patient�s blood was estimated using our designed biosensor and diagnosed for diabetes. Our instrumental results were found to be correlated with that measured using commercial glucometer. No significant deviation was observed.