THE COLLATION ONTOGENESIS BETWEEN P53 AND BCl-2 MARKERS IN THE PROGNOSIS OF BENIGN SURFACE EPITHELIAL TUMOURS OF OVARY

Maj(Dr) Ragini Thapa; Col(Dr) Prosenjit Ganguly; Capt(Dr)Amit Rajan

Volume : VI, Issue : XII, December - 2017

THE COLLATION ONTOGENESIS BETWEEN P53 AND BCl-2 MARKERS IN THE PROGNOSIS OF BENIGN SURFACE EPITHELIAL TUMOURS OF OVARY

Maj Dr Ragini Thapa, Col Dr Prosenjit Ganguly, Capt Dr Amit Rajan

Abstract :

Ovarian cancer is the most colloquial cause of death among all gynecologic malignancies and 90 % are of epithelial origin that undergo multiple genetic alterations during malignant transformation of these epithelial cells. Bcl-2 and p53 play a crucial role in tumor formation.The relationship between Bcl-2 and p53 gene expression in benign ovarian surface epithelial tumors gives the progression of a benign lesion undergoing metamorphosis to a malignant Form .The Study Design for our research article November 2012 – December 2015. All benign surface epithelial tumors of the ovary , biopsies as well as resected specimens are included in this study. Statistical Methods like Sensitivity, Specificity And Chi Square Testwere used. Routine H & E stained slides were studied under light microscope and after the cases diagnosed as tumours they are classified according to WHO classification into benign, borderline and malignant .All the cases were subjected to Bcl-2 and p53 immunohistochemical staining using Novocastra Lyophilized Mouse Monoclonal Antibody Bcl-2 and p53 protein for their expression.Strong expression of Bcl-2 in epithelium and stroma was seen in all benign and borderline tumors except few case each of mucinous cystadenoma.p53 expression was negative both in the epithelium and stroma in all benign tumors except few cases of mucinous cystadenoma (mild staining in the epithelium alone). Positive p53 in epithelium can be taken as reliable criteria of malignancy and Bcl-2 positivity can be relied upon to differentiate between benign , borderline and malignant tumors.Both the markers tend to show an inverse correlation in benign surface epithelial tumours of ovary.