Use of degenerate primers for molecular identification of begomovirus associated with yellow vein mosaic and leaf curl disease of okra in Meerut (UP)

Arvind Kumar; Gyanika Shukla; Sahadev Singh; Tanu Shri; Vijay Kumar; Mahendra Kumar

Volume : VII, Issue : VI, June - 2018

Use of degenerate primers for molecular identification of begomovirus associated with yellow vein mosaic and leaf curl disease of okra in Meerut (UP)

Arvind Kumar, Gyanika Shukla, Sahadev Singh, Tanu Shri, Vijay Kumar, Mahendra Kumar

Abstract :

The present study deals with the survey for the prevalence and collection of begomovirus infected okra samples from different regions of western Uttar Pradesh and molecular identification of the causal pathogen using begomovirus specific degenerate in primer through PCR.The result of positive PCR amplification of approximately 500bp from virus infected leaf sample of okra and absence of amplification in the healthy leaf tissue of okra clearly revealed the association of begomovirus with yellow vein mosaic virus and venation leaf curl disease of okra in western UP.