<p> PURPOSE: The purpose of this study is to compare fresh and cryopreserved amniotic membrane transplantation in various ocular surface disorders in terms of efficacy, cost effectiveness as well as simplicity of its use. MATERIAL AND METHODS: This study was a randomized, prospective, interventional and comparative clinical trial conducted at RIO, Allahabad which included 30 eyes with various corneal pathologies and divided in two groups of 15 eyes in each group(group I with fresh and group II with cryopreserved membrane). RESULTS: The overall success rate of 65.5% was observed with cryopreserved membrane showing a significant higher success rate of 86.5% as opposed to 40% in hypothermic grafts. The best results were seen in corneal ulcer(90%) and the worst in Steven Johnson syndrome(16.67%) CONCLUSION: A cryopreserved graft was seen to be superior to hypothermically stored graft.</p>