<p> Background: Rouviere’s sulcus is a lesser known, but important anatomic landmark for safe cholecystectomy and avoiding common bile duct injury during laparoscopic cholecystectomy. The identification this important landmark was done by Henry Rouviere in 1924. He used as a reference point to guide the commencement of safe liver dissection. The Rouviere’s sulcus is a fissure in the liver between the right lobe and caudate process and is clearly seen during a laparoscopic cholecystectomy during the posterior dissection in majority of patients. Material and Methods: The study was done in one hundred patients of gall chronic cholecystitis with cholelithiasis admitted for laparoscopic cholecystectomy. The standard four ports technique was used for laparoscopic cholecystectomy. Rouviere’s sulcus will be looked for behind the infundibulum and it will be noted if it is presented or absent. If present, it will be observed whether the sulcus is represented only as a scar or as a slit or as a deep sulcus. Observation was also done if the Rouviere’s sulcus was closed or open toward the porta hepatis. Results: Out of these 100 patients, the Rouviere’s sulcus was absent in 10% of patients, 7% had a scar, 24 patients had a slit, 59 patients had a deep sulcus. Conclusion: We recommend that identification of Rouviere’s sulcus should be done before dissection in Calot’s triangle is started as a safeguard to extra hepatic biliary injuries at porta hepatis.</p>