This paper focuses on the investigating the frustration level of D.T.Ed., (Diploma in Teacher Education) Teacher Trainees. 400 D.T.Ed., Teacher Trainees were taken as a sample. Out of these 129 male and 271 female teacher trainees were taken as sample. Statistical analysis was done by using descriptive and differential analysis. The result of the analysis reveals that the D.T.Ed. Teacher Trainees based on genders, optional subjects of study and parental qualification have average level of frustration.