A Rapid Latex Agglutination Test (Lat) for Qualitative  and Semi Quantitative Detection of Toxoplasma  Gondii Antibodies in Different Patient Categories

Sucilathangam G; Tina Jeyaraj; Anna T; Velvizhi G

Volume : III, Issue : VIII, August - 2013

A Rapid Latex Agglutination Test (Lat) for Qualitative and Semi Quantitative Detection of Toxoplasma Gondii Antibodies in Different Patient Categories

Sucilathangam G, Tina Jeyaraj, Anna T, Velvizhi G

Abstract :

This study emphasizes the use of a rapid latex agglutination test for qualitative and semi quantitative detection of Toxoplasma gondii antibodies in different patient categories. This prospective study was conducted on peripheral blood samples of HIV patients (72), pregnant women (50) and ocular chorioretinitis cases (7) those admitted at Tirunelveli Medical College Hospital, Tamil Nadu from April to September’ 2012. A total of 129 samples were subjected to Rapid Latex Agglutination Test (Toxogen) for qualitative and semi quantitative detection of Toxoplasma gondii antibodies. Out of 129 samples, eight samples (6.2%) gave positive results to Toxoplasma antibodies by Latex agglutination test (LAT).The test results show higher incidence of Toxoplasma antibodies in females (3.87%) than in males (2.3%) Out of 8 LAT positive cases, 4 were pregnant women, 3 were HIV patients and 1 presented with chorioretinitis. Out of 8, 3 were Positive with Toxoplasma IgM antibodies, 5 were IgG antibodies. Out of 8 LAT positive cases, 3 showed positive reaction at titre 1:16, 3 at 1:32 and 2 at 1:64. 2 gave positive for IgM antibodies and one for IgG antibodies at titres of 1:16 and 1:32 while one for IgM and IgG at 1:64 titre. The sensitivity and specificity of LAT test were 100% and 88.4% respectively