Volume : IX, Issue : VIII, August - 2019

Alstonia scholaris effects on peripheral human lymphocyte culture against MMS induced genotoxicity

Afsar Ali, M Sultan Ahmad

Abstract :

Use of medicinal plants in modern medicine for the prevention and treatment of cancer is an important aspect. For this reason, it is important to identify antitumor promoting agents present in medicinal plants commonly used by the human population, which can inhibit the progression of tumor. We used in vitro methods using chromosomal aberrations, sister chromatid exchange and replication index as markers, exposed by MMS as well as Alcoholic Extract of Alstonia scholaris in four increasing concentrations 150, 200, 250 and 300 µg/ml of culture) and of three different durations of 24, 48 and 72hrs in the presence as well absence of S9–mix. Alcoholic extracts of Alstonia scholaris reduced in vitro experiments i.e., it reduces chromosomal aberrations 39.62, 32.83, and 38.48% ±S.E at 24, 48, and 72hrs of exposure respectively; but when experiments were carried out in the presence of liver S9 fraction, these values were 45.31, 44.46, and 38.34% ± S.E respectively at <0.05 level, likewise it also reduces sister chromatid exchange from 7.70 to 4.20 ± S.E per cell and enhances replication index from 1.45 to 1.64 I. Alcoholic extracts of Alstonia scholaris significantly chromosomal aberrations and sister chromatid exchange and enhances replication index in vitro both of which were statistically significant at <0.05 level.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

ALSTONIA SCHOLARIS EFFECTS ON PERIPHERAL HUMAN LYMPHOCYTE CULTURE AGAINST MMS INDUCED GENOTOXICITY, Afsar Ali, M Sultan Ahmad INDIAN JOURNAL OF APPLIED RESEARCH : Volume-9 | Issue-8 | August-2019


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