Assessment of genetic diversity in Colletotrichum capsici using Randomly Amplified Polymorphic DNA

Saket Kumar; Vineeta Singh; Major Singh

Volume : V, Issue : I, January - 2015

Assessment of genetic diversity in Colletotrichum capsici using Randomly Amplified Polymorphic DNA

Saket Kumar, Vineeta Singh, Major Singh

Abstract :

Present study was undertaken to estimate genetic diversity in Colletotrichum capsici causing anthracnose disease on chilli fruits, using Randomly Amplified Polymorphic DNA (RAPD). In this study collect 12 isolates of Colletotrichum capsici from different sites and grow in PDA media. After pathogenicity test fungal pathogens were inoculate in PD oth. Mycelium mat form that harvest after 14–21 days and DNA was isolate. RAPD analysis after PCR amplification. Six RAPD primers were use to amplify genomic DNA of 12 Colletotrichum capsici isolates. DNA fragments of various sizes ranging from 250 to 4000 bp were amplified. The isolates were divided into two major clusters based on dendrogram construction from UPGMA clustering. Some pathogen cluster were to be pathogenically diverse and the diversity seems to be due to continuous generation of molecular characterization. The analysis of pathogenic cluster according to geographic distribution of the pathogenic population.