Volume : III, Issue : VI, June - 2013

Bacterial Polygalacturonase (PG) Production from Agro Industrial Waste by Solid State Fermentation

Preeti G. Dharmik, Dr. Ashok V. Gomashe

Abstract :

Agro–industrial wastes are mainly composed of complex polysaccharides that might serve as nutrients for microbial growth and production of enzymes. The aim of the current investigation was to study polygalacturonase (PG) production by Bacillus licheniformis cultured on media supplemented with the agro industrial waste (Potato peels). Total 40 bacterial isolates were isolated from clayed Potato peels collected from different restaurants in Nagpur district, MS, India. All bacterial isolates were then screened for their ability to produce polygalacturonase using potato peels by Solid state fermentation (SSF). Results showed that all of the isolates were found to have appreciable Pectinolytic ability of which 15 isolates showed good polygalacturonase producing potential using agro industrial waste at 370 C by pectin cleå zone. Five bacterial isolates were found to exhibit a higher polygalacturonase production by attacking potato peel. The bacterial isolates were identified on the basis of cell shape, cell arrangement, and relation to oxygen and physiological and biochemical tests as Bacillus licheniformis. The optimum incubation period observed was 96hrs at 6.6 pH. Out of all the nitrogen sources tested, Peptone, Urea and KNO3 gave promising results. These results suggesting that, the polygalacturonase was produced from cheap raw material under solid state fermentation under all optimal conditions

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Preeti G. Dharmik, Dr. Ashok V. Gomashe Bacterial Polygalacturonase (PG) Production from Agro Industrial Waste by Solid State Fermentation Indian Journal of Applied Research, Vol.III, Issue.VI June 2013

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