Volume : X, Issue : IV, April - 2020

BACTERICIDAL EFFECT OF PHYTOCONSTITUENTS OF MEDICINAL PLANTS

Shakti, Resal Raj

Abstract :

Introduction: The antimicrobial chemicals create health hazards for all organisms and the remedy to the health hazards is switching to bio–ingredients such as phytoconstituents of plants as bactericidal agents. This research analyses the useful effect of phytoconstituents of medicinal plants as bactericides. Methods: Leaves of Withania sominifera, Azadirachta indica, Solanum virginianum, and fruits and rhizome of Solanum virginianum and Alocasia odora were used to extract the phytoconstituents for the inhibition of bacteria, Clostridium sp, Pseudomonas andropogonis, Bacillus sp, Pseudomonas cichorii. The morphogenesis of growing pathogen in Nutrient oth was used to identified and measured the growth using spectrophotometer. Results: For the extraction of phytoconstituents from medicinal plants, 80% acetone as solvent for the extraction may be suitable. All bacterial species on study can be inhibited easily by 20mg/ml concentrations of phytoconstituents of Withania somnifera leaves, Azadirachta indica leaves, Solanum virginianum and fruit and seeds of Alocasia odora rhizome. For a minimum concentration of phytoconstitutents, 5mg/ml or 10 mg/ml is effective for the inhibition. Water as solvent for extraction of phytoconstitutents for bactericidal is ineffective. Bacillus sp. can be inhibited easily by all concentrations and by all medicinal plants on study. Clostridium sp. can be inhibited by using both, Rhizome of Alocasia odora and seed of Solanum virginianum, both in 80% acetone as solvent. Conclusion: The extracted concentration of phytoconstituents (5mg/ml, 10mg/ml and 20mg/ml) of medicinal plants in this study and the concentration of solvents (50% and 80% acetone and water) used for extraction of phytoconstituents for the inhibition of Pseudomonas sp., Clostridium sp. and Bacillus sp. vary with concentration of the phytoconstituents and concentration of the solvent used for the extraction.

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Article: Download PDF    DOI : 10.36106/ijar  

Cite This Article:

BACTERICIDAL EFFECT OF PHYTOCONSTITUENTS OF MEDICINAL PLANTS, Shakti, Resal Raj INDIAN JOURNAL OF APPLIED RESEARCH : Volume-10 | Issue-4 | April-2020

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