Bioprocessing of Areca husk in solid  state fermentation for cellulase production  using Trichoderma viride

Bhagyamma; Sharad S; Shriganesh G; Reshma S.V

Volume : I, Issue : III, December - 2011

Bioprocessing of Areca husk in solid state fermentation for cellulase production using Trichoderma viride

Bhagyamma, Sharad S, Shriganesh G, Reshma S. V

Abstract :

Solid–state fermentation (SSF) is an alternative to submerged fermentation for production of antibiotics, enzymes, biofuel, etc. Cellulases are a complex enzyme system, comprising endo–1,4–bD–glucanase (EC–3.2.1.4), exo–1,4–b –glucanase (exocellobiohydrolase, EC–3.2.1.91) and b –Dglucosidase (EC–3.2.1.21). Cellulases find applications in areas like textile industry, retting process, for the production of feed, fuel etc. This actual waste, Areca catechu husk from agriculture was used as a nourishing base by Trichoderma viride in SSF for cellulase enzyme production. The optimum growth was seen in 5 day culture with inoculums size 5% on the areca husk particles of 180 microns and media of 5.75 pH. The enzyme was extracted and its activity was found out to be 500 mg/ml/min. The results demonstrated that areca husk can be used as an inexpensive source (carbon) for industrial production of cellulase enzymes in solid state fermentation by Trichoderma viride.