Characterization of Adipogenic Differentiation of  Mesenchymal Stem Cell Derived from Mice Adipose  Tissue

Zahran F; El-Deen IM; Hamed S; EL-Shenawy A

Volume : III, Issue : VII, July - 2013

Characterization of Adipogenic Differentiation of Mesenchymal Stem Cell Derived from Mice Adipose Tissue

Zahran F, El Deen Im, Hamed S, El Shenawy A

Abstract :

Mesenchymal stem cells (MSCs) isolated from adipose tissue (AT) possess the capacity for self–renewal and the potential for multilineage differentiation, in the present study we characterized the adipogenic differentiation of mesenchymal stem cells derived from C57BL/6 mice adipose tissue. This is characterized by gene expression in different days during the process (C/EBP, PPAR–γ, LPL, FABP4, and B–Actin) which showed different stages of differentiation, although by stained the cells with Oil Red O stain which is specific for adipocyte and read absorbance for stained cells, so these results support that adipose tissue is a good alternative source for mesenchymal stem cells (MSCs) and show that there are specific genes for adipogenic differentiation which play vital role in differentiation.