Development of technique for efficient in vitro propagation of Jatropha curcas L.from nodal explants.

Dr. Kumari Anjali; Ritika Kumari; Dr. L.N.Shukla

Volume : V, Issue : I, January - 2015

Development of technique for efficient in vitro propagation of Jatropha curcas L.from nodal explants.

Dr. Kumari Anjali, Ritika Kumari, Dr. L. N. Shukla

Abstract :

Micropropagation technique is being applied for the production of large number of plants within a limited period and space. Therefore, this technique is being applied to generate large number of planting material with uniform genotype. In the present work multiple shoots on the nodal explants have been raised in MS basal medium supplemented with seven different concentrations viz., (0.5– 3.5) mg/l of BAP and 1.0 mg/l kinetin +0.5 mg/l IBA . Out of the above concentration , BAP 3.0 mg/l + KN 1.0 mg/l + 0.5 mg/l IBA was found more suitable than any other concentration of the similar combination or any other concentration of BAP alone or BAP + IBA. Induction of shoots were noted after 18th day of inoculation. These shoots were excised and sub cultured on the same medium. Denovo initiation of multiple shoots was observed after three weeks of the inoculation in sub culture. Half strength MS medium supplemented with IBA 3.0 mg/l + IAA 0.5 mg/l was found more suitable in which the average number (6–8) of roots were found. Well developed and rooted plants were used for hardening in artificial polychamber. They were planted in polybag and pots containing 1:1:1(Soil +Sand+Vremiculate) and the survival rate was 72%.