Effect of amikacin over biofilm formation of carbapenam resistant Pseudomonas aeruginosa on urinary catheters.

JayanthiS; Jeya M; T.Sundararaj

Volume : V, Issue : IX, September - 2015

Effect of amikacin over biofilm formation of carbapenam resistant Pseudomonas aeruginosa on urinary catheters.

Jayanthis, Jeya M, T. Sundararaj

Abstract :

Pseudomonas aeruginosa is a clinically troublesome Gram negative pathogen. Pseudomonas aeruginosa generally demonstrates resistance to various antimicrobial agents. Biofilm formation occurs in these indewelling devices leading to morbidity and mortality among the hospitalized patients. Any antibiotic or enzymes should be capable of inhibiting the biofilm formation, ready to detach the biofilm formed or capable of increasing the susceptibility of these biofilm producing isolates. In our study, in vitro effect of amikacin over the biofilm produced by the carbapenem resistant isolates was carried out and the impact produced by the drug over the biofilm producer was analysed. The multidrug resistant strains of Pseudomonas aeruginosa, resistant to carbapenem drugs were included in the study. Three different strains 3, 9 and 21(strain number) of MBL positive strains of P.aeruginosa were taken for the study of biofilm forming characteristics on urinary catheter. Once the biofilm formation of the MBL producing P.aeruginosa is confirmed by the quantitative viable count method, the isolates are subjected to the biofilm prevention by amikacin. Significant decrease in biofilm formation as indicated by the CFU was seen on 5 days and 10 days of observation. Thus amikacin–agarose coated catheter tips significantly reduced the biofilm formation.