Effect of Furadan on Protein Content, Reduced Glutathione and Lipid Peroxidation Level on the Muscle of Bufo Melanostictus Schneider, 1799

Puspanjali Parida; Harapriya Mangaraj; Sudipta Chakrabarti

Volume : IV, Issue : V, May - 2014

Effect of Furadan on Protein Content, Reduced Glutathione and Lipid Peroxidation Level on the Muscle of Bufo Melanostictus Schneider, 1799

Puspanjali Parida, Harapriya Mangaraj, Sudipta Chakrabarti

Abstract :

Bufo melanostictus (n=20) of various sizes (body weight: 90g– 120g) were divided into four groups of 5 anmals each. Group I (control) animals received distilled water; Group II–IV (experimental) treated orally with 3μl per 1gm of body weight of furadan (0.005mg of furadan dissolved in 1ml of acetone). The treated animals were sacrificed after the time intervals of 24 hour, 48 hour and 72 hour (Group II–IV) whereas the control animal was sacrificed immediatetly (0h) and the liver was dissected out and kept at 00C. The protein content, reduced glutathione level (GSH) and lipid peroxidation (LPX) level were measured in the liver of B. melanostictus in both control and experimental group. Variation of different parameteres of liver were observed at different time intervals. On the basis of the results, It is concluded that furadan even at low dose altered the biochemical parameters and induces oxidative stress