Volume : III, Issue : VII, July - 2014

Effect of Substrate Concentration on the Activity of Cellulase produced by Aspergillus flavus

I. F. Okonkwo

Abstract :

The purpose of this research is to determine the optimum concentration of various substrates, including carboxymethyl cellulose (CMC), filter paper and cotton wool, for inducing the highest cellulase activity in Aspergillus flavus. Substrates for cellulase activity assays can be divided into two categories, based on their solubility in water. CMC is soluble in water while cotton linter and Whatman No. 1 filter paper are insoluble. Based on the result of the research, there were increase in cellulase activity as the substrate concentrations increased and were highest at 1 % concentration in all the substrates but the rate of increase in activity was highest in cotton wool and least in CMC. The enzyme also showed normal Michaelis Menten behavior with CMC, filter paper and cotton wool substrates. However, cotton wool is a better substrate, than filter paper and CMC. This is evident from the lower Km and higher Vmax0.7 mg/ 0.08 for cotton wool, and thus is recommended for use in industrial processes involving Aspergillus flavus cellulase system 

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

I. F. Okonkwo Effect of Substrate Concentration on the Activity of Cellulase produced by Aspergillus flavus Indian Journal of Applied Research, Vol.4, Issue.7 July 2014


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