Volume : IV, Issue : IV, April - 2014

Evaluation of Renal Tubulointerstitial Injury after Mesenchymal Stem Cells Treatment

Zahran F, El Ghareb M, Hamed S, Nabil A

Abstract :

 Purpose Mesenchymal stem cells (MSCs) hold apromise for the treatment of renal disease, While MSCs have been shown to accelerate renal recovery and prevent acute renal failure in multiple disease models, the effect of MSC therapy on chronic obstruction–induced renal fiosis has not previously been evaluated. Materials and Methods 60 C57Bl/6 male mice underwent injection of bone marrow–derived stem cells (MSCs) immediately prior to sham operation or induction of left ureteral obstruction (UUO). One or 2 weeks later, the kidneys were harvested, fixed in 10% buffered formalin, and embedded in paraffin for morphological studies. Serum creatinine, Blood urea nitrogen (BUN) and uric acid were measured in all mice involved in this study. Results There was a significant decrease in serum creatinine, Blood urea nitrogen (BUN) and uric acid in all MSC groups compared to all control groups (p < 0.001). Kidney specimens obtained from mice treated with MSC before operation showed regeneration of the renal tubular cells, less tubular atrophy, very mild interstitial fiosis and normal blood vessels. While kidney specimens obtained from mice treated with MSC (1Week)and (2 Weeks) after induction of UUO showed mild shrinkage of vascular tuft with normal basement memane and cellularity, marked tubular atrophy with cast formation, mild interstitial fiosis and normal blood vessels. Conclusions Bone marrow–derived MSCs provide protection against renal tubulointerstitial injury induced by ureteral obstruction.

 

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Zahran F, El-Ghareb M,Hamed S, Nabil A Evaluation of Renal Tubulointerstitial Injury after Mesenchymal Stem Cells Treatment Indian Journal of Applied Research, Vol.IV, Issue. IV


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