In Vitro Assessment of Antagonistic Activity of  Trichoderma Viride and Trichoderma Harzianum  Against Pathogenic Fungi

Shaikh Farah T.; Sahera Nasreen

Volume : III, Issue : V, May - 2013

In Vitro Assessment of Antagonistic Activity of Trichoderma Viride and Trichoderma Harzianum Against Pathogenic Fungi

Shaikh Farah T. , Sahera Nasreen

Abstract :

Trichoderma is a promising candidate for the biological control of plant pathogenic fungi. In an attempt to develop biocontrol system for management of different fungal vegetable diseases, Trichoderma viride and Trichoderma harzianum were evaluated for their antagonistic activity against five different pathogens in vitro. Both Trichoderma spp were strongly antagonized with five different pathogenic fungi viz. Colletotrichum lindemuthianum (C.o), Fusarium oxysporum (F.o), Rhizoctonia solani (R.o), Alternaria solani (A.s), Fusarium solani (F.o) in dual culture assay. T. viride gave maximum inhibition of mycelia growth to all pathogenic fungi (67.45%), where as T. harzianum showed (63.89%) inhibition of mycelia growth. Volatile metabolites produced by T. viride exhibited highest growth inhibition rate (37.85%) followed by T. harzianum (37.78%). The Metabolites released from T. harzianum, T.viride were tested in culture medium against all pathogens. Cell free metabolites of T. viride, T. harzianum inhibited the growth of all five pathogens in vitro and appeared to be fungicidal in its activity.