In vitro plantlet regeneration from tendril explants of spine gourd (Momordica dioica Roxb. ex. wild)

S.Raju; Ravi Chithakari; Md. Mustafa

Volume : IV, Issue : V, May - 2014

In vitro plantlet regeneration from tendril explants of spine gourd (Momordica dioica Roxb. ex. wild)

S. Raju, Ravi Chithakari, Md. Mustafa

Abstract :

The effect of amino acids in combination with cytokinin was observed for the maturation and regeneration of emyogenic callus in spine gourd. Highest percentage (80%) emyogenic callus was obtained on MS medium fortified with 2.0 mg/L each of 2, 4–D and BAP in tendril explants. Maximum numbers of shoots (8 shoots/ emyogenic calli) were observed on MS medium supplemented with 4.0 mg/L BAP in combination with 2.0 mg/L L–glutamic acid from emyogenic calli derived from tendril explants. Addition of the amino acid L–glutamic acid provides plant cells with an immediately available source of nitrogen, which generally can be taken up by the cultured cells more rapidly than inorganic nitrogen. All shoots were rooted on MS medium augmented with IBA (3.0 mg/L). The regenerated plantlets were acclimatized in plastic pots and transferred to field condition with 70% survivability