Volume : IV, Issue : XII, December - 2014

In–Vitro multi enzymatic degradation process of Agrowastes in the production of Bioethanol

Prasad. M. P

Abstract :

In this present work, bioethanol was produced by using 15 ready and cheaply available agricultural raw materials. The biomass used are cassava, fruit pulp, rice extract, sweet potato, potato, sugar cane beet, saw dust, coconut pith, groundnut waste, rice straw, rice husk, leaf litter, wood bark, maize husk and waste paper. To produce ethanol from biomass two key processes were followed, first the starch or hemicellulose and cellulose portions of the biomass were oken down in to simple sugars through a process called saccharification. Second, the sugars are fermented to produce ethanol. Two enzymes were used for the hydrolysis of the biomass namely amylase from Aspergillus niger and cellulase from Trichoderma viride. Cellulase was used for the hydrolysis of cellulose and hemicellulose and amylase is used for the hydrolysis of starch present in the raw materials. Fermentation of the hydrolyzed samples was done using Sacharomyces .sp. The fermented product was purified by primary distillation process at 80°C and the fraction is collected. The ethanol is then determined by specific gravity method.  

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Prasad.M.P In-Vitro Multi Enzymatic Degradation Process of Agrowastes in the Production of Bioethanol Indian Journal of Applied Research, Vol.4, Issue : 12 December 2014

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