Isolation and characterisation of endophytic bacteria from Eleusine coracana from the fields of Darbhanga

Shikha Shirsh; Jaykar Jha

Volume : X, Issue : III, March - 2020

Isolation and characterisation of endophytic bacteria from Eleusine coracana from the fields of Darbhanga

Shikha Shirsh, Jaykar Jha

Abstract :

Endophytic bacteria hold an assurance for better plant growth and crop production.But the potential of endophytic bacteria has not been studied with reference to the coarse grain so far.Finger millet is a rain–fed crop which is highly nutritious and can be seen as a mean to ensure food security and agricultural sustainability in the long run.Endophtyic bacteria were isolated from roots,stems and leaves of this crop collected from different areas of the district Darbhanga.The samples were surface sterilised and isolated by culturing on NA medium.The endophytic bacterial isolates were then analysed morphologically using Gram’s staining and at molecular level using PCR amplification.It was found that 52.17% (24) and 47.83% (22) were Gram positive and Gram negative respectively. Out of total endophytic bacterial isolates, 7 isolates were detected as Pseudomonas sp .Thus,it can be concluded that endophytic bacteria hold an assurance for better plant growth and crop production.