Isolation and Characterization of Phosphate  Solubilizing Pseudomonas Sp. from Rhizospheric Soil  of Rice

Manidipa Roy; G.D.Sharma; Ch.V. Ramana

Volume : III, Issue : IX, September - 2013

Isolation and Characterization of Phosphate Solubilizing Pseudomonas Sp. from Rhizospheric Soil of Rice

Manidipa Roy, G. D. Sharma, Ch. V. Ramana

Abstract :

Phosphorus (P) is an essential macronutrient for the growth of plants. However , in most soils a large portion of phosphorus becomes insoluble and therefore, unavailable to plants. Thirty one Pseudomonas sp. were isolated from rhizospheric soil of rice. Out of thirty one nine isolates were able to solubilize Ca3(PO4)2 on Pikovskya’s agar medium. A total six sp. of phosphate solubilizing fluorescent pseudomonads which showed fluorescence under UV radiation (λ=365nm) viz. Pseudomonas aeruginosa, P. fluorescens, P. putida and P. plecoglossicida and three species of phoSphate solubilizing non–fluorescent pseudomonas were isolated. Isolate MGR38 was tentatively identified as Pseudomonas fluorescens which showed 83.33% efficiency in phosphate solubilization and the other isolate MGR31, MGR37, MGR39, MGR40, MGR41, MGR42, MGR43 and MGR44 were showed 33.33, 57.14, 50, 50, 16.16, 40, 60 and 80 % efficiency of phosphate solubilization respectively