Volume : IV, Issue : II, February - 2014

Isolation and Screening of Some Filamentous Fungi with Various Trace Metals

Sharma, G. D, Hijri Mohamed, Dhritiman Chanda, D. K. Jha

Abstract :

Trace metal (TM) pollution of soil is a worldwide problem threatening the quality of human life and a proper environment. We investigated fungal diversity of trace metal polluted site contaminated with paper mill effluent in India. Fourteen fungal strains were identified using ITS of rDNA belonged to Aspergillus, Penicillium, Fusarium, Cunninghamella, Simplicilillium, Trichoderma, Rhizomucor, Cladosporium and Hypocrea and subsequent screening was carried out to assess their TM tolerance in in vitro cultures. The results revealed that the majority of the isolates were tolerant to Ni, Cu, Zn whereas only Penicillium sp. and Aspergillus sp. were able to grow in Cd amended medium. The level of tolerance depended on the fungal isolate and the site of its origin. Minimum inhibitory concentrations (MIC) for Ni, Cu, Zn and Cd were also determined among the all tested fungal isolates. Overall, Aspergillus, Penicillium, Rhizomucor, Trichoderma and Fusarium isolates showed a strong growth in different concentrations of TM. Their MIC ranged between 20 and 25 mM for Zn, 15 and 20 mM for Ni and Cd. Some of these fungal isolates showed a high potential for further investigations regarding the mechanisms of TM tolerance and their use in mycoremediation to clean up TM polluted soil.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Sharma, G. D, Hijri Mohamed, Dhritiman Chanda, D.K. Jha Isolation and Screening of Some Filamentous Fungi with Various Trace Metals Indian Journal of Applied Research, Vol.IV, Issue. II

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