Isolation, Identification, Enzymatic and Molecular Characterization of Intestinal Bacteria of Goldfish (Carassius Auratus) and its Role on Growth

D.Suganya.; M.R.Rajan; P.Sivakumar

Volume : III, Issue : VII, July - 2014

Isolation, Identification, Enzymatic and Molecular Characterization of Intestinal Bacteria of Goldfish (Carassius Auratus) and its Role on Growth

D. Suganya. , M. R. Rajan, P. Sivakumar

Abstract :

The present study deals with the isolation, identification, enzymatic and molecular characterization of intestinal bacteria of goldfish and its role on growth for a period of 45 days. Five distinct colonies such as GB1 (Pseudomonas sp.,), GB2 (Streptococcus sp.,), GB3 (Bacillus sp.,), GB4 (Proteus sp.,) and GB5 (Viio sp.,). were isolated from the intestine of gold fish and based on biochemical tests, enzymatic productivity and molecular characterization new bacterium was identified as Pseudoxanthomonas kalamensis and mass multiplied. Six different feeds having different concentration of bacterial sp. such as feed I (Control), Feed II (1ml), Feed III (2ml), Feed IV (3ml), Feed V (4ml), and Feed VI (1ml yeast) were prepared and given to gold fish for a period of 45 days. The feed consumption, feed conversion ratio, feed conversion efficiency, growth, percentage growth, relative growth rate, assimilation, metabolism, gross growth efficiency and net growth efficiency was higher in feed V