Volume : IV, Issue : II, February - 2014

Isolation, Partial Purification and Biochemical Characterization of Enterocin Producing Enterococci

J. H. Anandani, Dr. Z. H. Khan

Abstract :

Bacteriocins are bacterial proteins or peptides that inhibit strains and species that are uaually but not always closely related to the producing bacteria.Enterocins are novel bacteriocin produced by enterococcus species. Many enterococcus species from various ecosystems were characterized as antagonists of oad range of pathogens. Broad spectrum activities against prominent pathogen make it an issue of medical interest. The ability to produce such biocompound may play a role in providing an ecological advantage on non bacteriocin producer strains. Thus, 40 strains of enterococci were isolated from urine samples of UTI patients. These strains were identified to species E.faecalis and E.hirae. Enterocin activity of all the 40 tested isolate was checked against indicator strain on Tripticase Soy Agar by agar well diffusion assay. From these 26 isolates displayed strong inhibitory activity against indicator strain. The purified enterocin was found to be active against all Gram positive and Gram negative bacteria.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

J.H.Anandani, Dr.Z.H.Khan Isolation, Partial Purification and Biochemical Characterization of Enterocin Producing Enterococci Indian Journal of Applied Research, Vol.IV, Issue. II

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