Volume : III, Issue : V, May - 2013

Mimicking Microaerophilic/Anaerobic Environment for Culture of Anaerobic Bacteria Using Oxygen Scavengers in Agar Well Technique

Harsh Nitin Dongre, Alok Goel

Abstract :

A cheap and simple method for culture of anaerobic bacteria using oxygen scavengers in agar well technique is described. A recognized bacterial strain was taken which was known to be anaerobic by nature. In order to replicate the natural microaerophilic growth environment, the bacteria were cultured in a modified media with oxygen scavengers in agar well technique. It was found that this method successfully simulated the natural microaerophilic growth of the bacteria which is difficult to achieve in the laboratory. This technique will be very useful for microbiological studies on growth and culture of anaerobic bacteria and to ensure that organisms are well protected from oxygen.A cheap and simple method for culture of anaerobic bacteria using oxygen scavengers in agar well technique is described. A recognized bacterial strain was taken which was known to be anaerobic by nature. In order to replicate the natural microaerophilic growth environment, the bacteria were cultured in a modified media with oxygen scavengers in agar well technique. It was found that this method successfully simulated the natural microaerophilic growth of the bacteria which is difficult to achieve in the laboratory. This technique will be very useful for microbiological studies on growth and culture of anaerobic bacteria and to ensure that organisms are well protected from oxygen.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Harsh Nitin Dongre, Alok Goel Mimicking Microaerophilic/Anaerobic Environmentfor Culture of Anaerobic Bacteria Using Oxygen Scavengers in Agar Well Technique Indian Journal of Applied Research, Vol.III, Issue.V May 2013


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