Novel and Simple Method for in Vitro Production of Psoralen  via Cotyledonary Culture of An Endangered  Leguminous Taxon– Psoralea Corylifolia Linn.

SHAHNAZ  SUBHAN; M.Z. ABDIN

Volume : IV, Issue : IV, April - 2014

Novel and Simple Method for in Vitro Production of Psoralen via Cotyledonary Culture of An Endangered Leguminous Taxon– Psoralea Corylifolia Linn.

Shahnaz Subhan, M. Z. Abdin

Abstract :

First time, a novel and simple protocol has been successfully developed for in vitro production of psoralen through rapid and recurrent callus cultures from one week old cotyledon of Psoralea corylifolia Linn. Amongst the various auxins and cytokinins tried alone or in combinations, 10 µM 2,4–dichlorophenoxyacetic acid (2, 4–D) supplemented to Gamborg et al. (1968) basal medium (B5) was optimum for inducing profuse amount of callus in cent percent cultures of cotyledon. The cotyledon calluses were subcultured continuously at an interval of 35–40 days on 5µM 2, 4–D medium up to two year and good amount of callus proliferation was achieved in cent percent cultures. However, some cultures were maintained under optimum cultural condition on B5+5µM 2, 4–D+5µM BA with 0.5% polyethylene glycol (w/v) up to 3 months. These callus cultures showed the maximum psoralen content of 16.15 mg/g FW. Psoralen has been detected time to time from calluses of different time durations grown on different type of culture media under optimum cultural conditions through HPLC by using caliation curve of standard psoralen. Psoralen estimation was also done for mature seeds samples collected from various localities of central India