Optimization of Detoxification with Over Liming and Charcoal Treatment for Increasing the Fermentability of Cotton Stalk Hydrolyzate

Mirza Zaheer Baig; Smita M. Dharmadhikari

Volume : III, Issue : VII, July - 2014

Optimization of Detoxification with Over Liming and Charcoal Treatment for Increasing the Fermentability of Cotton Stalk Hydrolyzate

Mirza Zaheer Baig, Smita M. Dharmadhikari

Abstract :

Fermentability of lignocellulosic hydrolyzate decreases by retarding microbial fermentation due to presence of inhibitors released during hydrolysis. These inhibitors can be removed by applying proper detoxification process. Among different process, present study focuses on detoxification of cotton stalk acid hydrolyzate by over liming followed by activated charcoal treatment for removal of sugar byproducts i.e. furans (furfural and 5, hydroxy methyl furfural) and traces of lignin in the form of phenolic compounds for the purpose to increase the fermentability of hydrolyzate. In the present investigation, optimization was carried out by overliming up to various pH ranges from 7 to 12 while charcoal concentration was used from 1% to 5% percent. Finally, optimum concentration was achieved by overliming with pH 10 and 4% activated charcoal which causes 92.69% and 88.89% reduction in furans and phenolics respectively with 19.84% decrease in fermentable sugar concentration was observed.