Volume : III, Issue : VII, July - 2013

Screening and Characterization of Actinomycetes for Production of Anti–Leukemic L–Asparaginase

Dange V. U, . S. A. Peshwe

Abstract :

Asparaginase (L–asparagine amido hydrolase, E.C. 3.5.1.1) is an anti–neoplastic agent, used in the lymphoblastic leukaemia chemotherapy. However L–asparaginase from bacterial origin can cause hypersensitivity in the long–term used, leading to allergic reactions and anaphylaxis. The search for other asparaginase sources, like eukaryotic microorganisms like fungi, actinomycetes etc. can lead to an enzyme with less adverse effects.  Various microorganisms’ viz. bacteria, fungi, actinomycetes and yeast, have ability for production of L–asparaginase. In the present investigation actinomycetes have been screened for L–asparaginase production. Primary and secondary screening has been done for 10 actinomycetes cultures for L–Asparaginase production. Assay was carried out for selection of maximum L–Asparaginase producers. The isolates exhibited extracellular as well as intracellular activity. Amongst the screened actinomycetal cultures 60% actinomycetal cultures showed remarkable extracellular enzyme activity. The actinomycetal isolate Act10 showed 202.88 µM/min enzyme activity which was screened as maximum L–asparaginase producer which was used for characterization.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Dange V.U,. S.A. Peshwe Screening and Characterization of Actinomycetes for Production of Anti-Leukemic L-Asparaginase Indian Journal of Applied Research, Vol.III, Issue.VII July 2013

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