Volume : III, Issue : VI, June - 2013

Screening and cultural studies of Aspergillus niger for production of Glucose Oxidase

Gothoskar V. S, Dharmadhikari S. M.

Abstract :

From total 17 isolated fungi from varied microbial ecosystem the fungal strain Aspergillus niger strain–F–405–2 (Gene Bank Accession No.JQ388756.1) was selected based on highest glucose oxidase activity (4.86U/ml). The growth parameters of Aspergillus niger strain–F–405–2 were optimised for increased production of Glucose–oxidase. Enzyme activity was found to be enhanced at pH 5.0 (8.96 U/ml) and at temperature of 400C (8.69U/ml). Further during periodic monitoring of growth the highest enzyme activity was observed in logarithmic phase of growth after 48 hours of incubation and was found to be 1.78U/ml. In the presence of glucose as carbon source supplemented with sucrose the highest glucose activity was observed (6.31U/ml). Similarly enhanced glucose oxidase activity was observed in presence of ammonium nitrate supplemented with proteose peptone as source (6.2U/ml)

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Gothoskar V. S, Dharmadhikari S. M. Screening and cultural studies of Aspergillus niger for production of Glucose Oxidase Indian Journal of Applied Research, Vol.III, Issue.VI June 2013

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