Volume : IV, Issue : III, March - 2014

Screening the Antibacterial Actinomycetes From Saline Soil Of Vidarbha Region

A. A. Deshmukh, N. N. Vidhale

Abstract :

Antibacterial activity of Actinomycetes isolated from saline belt of Purna river basin which appears in Amravati, Akola and Buldhana district of vidarbha region has been studied. In primary screening, out of 147 actinomycete isolates 50 isolates (34.01%) showed an activity against 2 test bacteria such as Staphylococcus aureus and Escherichia coli by agar overlay technique. In secondary screening, out of 50 primary isolates 19 actinomycete isolates were proceeded for an antibacterial activity against Staphylococcus aureus (MTCC 7443), Bacillus subtilis (MTCC 441), Escherichia coli (MTCC 443) and Pseudomonas aeruginosa (MTCC 424) by agar well diffusion method. Nearly 78.94% isolates were found antibacterial to Staphylococcus aureus followed by 68.42% against Bacillus subtilis, 52.63% against Escherichia coli and 42.10% against Pseudomonas aeruoginosa. Similarly actinomycetes isolates KR4, N3, N8 and C6 showed antibacterial activity against all the test bacteria

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

A.A. Deshmukh and N.N. Vidhale Screening the Antibacterial Actinomycetes From Saline Soil Of Vidarbha Region Indian Journal of Applied Research, Vol.IV, Issue. III


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References :

Adyalkar, P.G. 1971. “Paleogeography, Sedimentation frame work and ground water potentiality of the Arid Zone of Western India”. Proc. on Symposium on Problems of India Arid Zone CAZRI. 4–17. Anansiriwattana W, Tanasupawat S, Amnuoypol S, Suwanborirux k 2006. Identification and antimicrobial activities of actinomycetes from soils in Samed Island, and geldanamycin from strain PC4–3. Thai. J. Pharm. Sci. 30: 49–56. Basilio,A., I.Gonzalez, M.F.Vicente, J.Gonochatequi, A. Cabelio, A.Gonzalez and O. Geneilloud. 2003. “Patterns of antimicrobial activities from soil actinomycetes isolated under different condition of pH and salinity”. J. of Applied Microbiol. 95: 814–823.Bergeys, Mannual of systematic bacteriology vol. 4. 1989. S. T. Williams, ed. Editonal board. Murray. Brenney holt Krieg. Moulder. Pfennig. Sneath. Staley and William.The Williams and Wilkins Comp.Batim.Charoensopharat K, Thummabenjapone P, Sirithorn P, Thammasirirak S. 2008. Antibacterial substance produced by Streptomyces sp. No. 87. Afr. J. Biotechnol. 7(9): 1362–1368. Chougule, V.V.and A.M. Deshmukh. 2006. “Studies on actinomycetes from saline soils of Sangli district”. Ph.D Thesis, Shivaji University Kolhapur. Mohmoud, S.A.Z., S.M. Taha, A.E.L.Damety and F.Anter. 1969. “The effect of some soil amendments on chemical and microbiological properties of an alkaline soil”. Plant and Soil. 80(1): 1–4. Mustafa Oskay, A. Usame Tamer and Cem Azeric. 2004. “Antibacterial activity of some actinomycetes isolated from farming soil of Turkey”. African J. of Biotechnol. 3(9): 441–446.Okami Y. and Hotta K. 1988. editors, Goodfellow M., Williams S.T. and Mordarski M. Academic Press Inc, New York. 33–67. P. A. Mary Helen, J. A. Johnson1 and T. Citarasu. 2012. “Screening of Antibiotic Producing Actinomycetes from Streams”. J. Chem. Bio. Phy. Sci. Sec. B.2 (3): 1363–1370. Pandey B., Ghimire P. and Agrawal V.P. 2004. International Conference on the Great Himalayas: Climate, Health, Ecology, Management and Conservation, Kathmandu, Organized by Kathmandu University and the Aquatic Ecosystem Health and Management Society, Canada. Sagare B.N., S. K. Thakare, B.A. Sonune. 2000. “Saline soil of Purna river basin of Vidarbha region”. Department of Agricultural chemistry and soil sciences, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.) Extension report 2000 (84) Supanekar,S.V. and P.L.Patil. 1999. “Impact of soil salinity on microflora and ground water pollution in Sangli district”. Ph.D. Thesis Shivaji University, Kolhapur. Taso, P.H., C.Leben and G.W.Keitt. 1960. An enrichment method for isolating actinomycetes that produce diffusible antifungal antibiotics. Phytopathology. 50: 88–95. Williams, S. T. and Cr oss, T. 1971. Actinomycetes. Applied microbiology 4, Academic Press.