Volume : III, Issue : V, May - 2013

Statistical Evaluation of NP–HPTLC and RP–HPTLC for Estimation of Triflusal in Bulk and in Capsule Formulation

Jineetkumar B. Gawad, Vijay K. Patil

Abstract :

A simple, rapid, selective and sensitive NP– HPTLC and RP–HPTLC/ densitometry method was developed and validated for the estimation of triflusal. For normal phase Toluene: Methanol: Triethylamine (8: 2: 0.5 v/v/v) and methanol: water: triethylamine (6:4:0.5 v/v) as a mobile phase in normal and reverse phase respectively. Both analyses were scanned with a camag scanner–3 at 314 nm. In normal phase and reverse phase Rf values 0.40 ± 0.02 and 058 ± 0.02 respectively. Linearity was studied in the concentration range of 1000 to 6000 ng band–1 for normal and 10000 to 35000 for reverse phase with correlation coefficient 0.999 for both methods. The result obtained shown that the method best fits for estimation of drug in capsule formulation and thus can be used for its routine analysis. The newly developed method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The analysis of variance (ANOVA) and Student’s t–test were applied to compare and correlate the results of Triflusal determination in dosage form by means of normal and reverse phase thin layer liquid chromatographic method.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Jineetkumar B. Gawad, Vijay K. Patil Statistical Evaluation of NP-HPTLC and RP-HPTLC for Estimation of Triflusal in Bulk and in Capsule Formulation Indian Journal of Applied Research, Vol.III, Issue.V May 2013


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