Studies on micropropagation of gerbera (Gerbera jamesonii  Bolus)

Bhavya Bhargava; Dr. B. S. Dilta; Y.C. Gupta; S.R. Dhiman; Manju Modgil

Volume : III, Issue : XI, November - 2013

Studies on micropropagation of gerbera (Gerbera jamesonii Bolus)

Bhavya Bhargava, Dr. B. S. Dilta, Y. C. Gupta, S. R. Dhiman, Manju Modgil

Abstract :

A study was carried out on micropropagation of Gerbera jamesonii Bolus cv. ‘Harley’. Capitulum explants were tried to develop protocol for organogenesis and plant establishment during 2009–10. The earliest culture establishment of capitulum sections was obtained on modified MS medium supplemented with Benzyl–6–adenine (4 mg/l) and Indole–3–acetic acid (0.5 mg/l). The type, concentration and combination of cytokinin also showed profound effect on multiplication. Better proliferation of quality shoots was obtained on modified MS medium supplemented with combination of BA and kinetin. The optimum numbers of quality shoots were obtained on MS medium containing 4 mg/l BA and 2 mg/l kinetin. For in vitro rooting, half strength MS medium supplemented with 2 mg/l Indole–3–butyric acid was found best.