The Biochemical Efficacy of Insulin Resistance on Sex  Hormones in Obese Male Rats

Gamal. E. Abdelhameed; Sherif. Y. Saleh; Mohamed. E. Rashad; Ibrahim. A. Ibrahim

Volume : III, Issue : VI, June - 2013

The Biochemical Efficacy of Insulin Resistance on Sex Hormones in Obese Male Rats

Gamal. E. Abdelhameed, Sherif. Y. Saleh, Mohamed. E. Rashad, Ibrahim. A. Ibrahim

Abstract :

OBJECTIVE: Insulin resistance has been associated with low total and free testosterone and increased the risk for type2 diabetes in males. Truncal obesity, a case accompanied by increased insulin resistance, is also associated with low total and free testosterone levels. In this study we evaluated if the insulin resistance primarily related to low total and free testosterone in obese males, and the effect of treatments for hypercholesterolemia on the levels of sex hormones. RESEARCH DESIGN AND METHODS: Fifty male rats, their weights between 150–200 gm. were divided into5groups: one as control fed on low fat diet, groups (2, 3, 4, and 5) fed on high fat diet. Group 2 serve as obese without treatment addition. Different treatments for hypercholesterolemia were added to groups (3, 4, and5) as Ezetimibe, Simvastatin, and Simvastatin+Ezetimibe respectively .Hormonal parameters include: Total testosterone, free testosterone, FSH, and insulin were measured. Also biochemical parameters include: FBS and cholesterol were measured. Insulin sensitivity index was measured by was calculated using homeostasis model assessment (HOMA) formula. RESULTS: Low level of total testosterone was associated with obesity (0.9 ±0.07, and 2.98 ±0.07 in control), also free testosterone was associated with it (0.3 ±0.03, and 0.75 ±0.02 in control).Insulin sensitivity index was negatively correlated with obesity (r = – 0.425, p= 0.05).And have a positive correlation with testosterone (r =0.540, p=0.001) in obese males. Significant improvement in total testosterone and free testosterone level found among the treated groups when compare with obese rats but still lower than control. CONCLUSIONS: We conclude that insulin resistance associated with lowering in total and free testosterone depends on the obesity. So obesity has negative relations with total and free testosterone, and positive with insulin resistance. We also conclude that combination of simvastatin + ezetimibe record the lowest level of cholesterol and FBS and highest improvement in insulin sensitivity index, total, and free testosterone levels among treated groups.