Objective: This study was done to assess the alteration in liver function test following laparoscopic cholecystectomy post–operatively. Material and Methods: A prospective study conducted on 182 patients reported in surgery OPD Patna Medical College and Hospital, Patna from July 2017 to June 2018 with cholelithiasis. All the patients underwent laproscopic cholecystectomy. Both pre–operative and post–operative liver function test was done. Results: Out of 182 patients, 46 were male and 136 were female patients. The mean age was 32.3 years. The level of serum ALT and AST increased significantly within the first 24 hours following operation, AST showed mild increase in 36% of the cases while ALT increased in 12% of the cases. Total bilirubin and direct bilirubin showed a slight increase within the first 24 hours following operation in 2% of the patients. Alkaline phosphatase increased in 10% of the patients. Conclusion: Laproscopic Cholecystectomy lead to transient but reversible significant hepatic enzymes alterations. These alterations are self limited and return to reference values within 7 days of operation. The cause of alteration might be liver tractions, electrocoagulation’s and manipulation of duct.