Volume : IV, Issue : V, May - 2015

ANTIFUNGAL ACTIVITY OF DOUBLE HEADED PROTEASE INHIBITORS FROM THE SEEDS OF ABELMOSCHUS MOSCHATUS

Muni Kumar Dokka, Siva Prasad Davuluri

Abstract :

 The main aim of the study was to investigate the antifungal potential of double headed protease inhibitors (AMTIIII & AMTI–IV) isolated from Abelmoschus moschatus seeds on selected pathogenic fungal strains. Both the inhibitors have been purified to homogeneity following the conventional methods of protein purification and they exhibited strong antitryptic and  antichymotryptic and moderate antielastase activities. Apparent molecular weights of AMTI–III & AMTI–IV were determined to be 20.8 kDa  and 20.2kDa respectively following gel filtration and SDS–PAGE. When tested in vitro, both the inhibitors significantly affected the growth  of Candida albicans, Candida tropicalis, Asperigillus flavus, Saccharomyces cerevisiae,Candida glaata and Asperigillus niger and were  ineffective against Fusarium oxysporum, Alternaria alternata, Mucor indicus and Penicillium chrysogenum. Results obtained in this study  suggest that double headed protease inhibitors may serve as excellent candidates for the development of novel antimicrobial agents against  human pathogenic diseases.

Keywords :

Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

Muni Kumar Dokka, Siva Prasad Davuluri ANTIFUNGAL ACTIVITY OF DOUBLE HEADED PROTEASE INHIBITORS FROM THE SEEDS OF ABELMOSCHUS MOSCHATUS International Journal of Scientific Research, Vol : 4, Issue : 5 May 2015


Number of Downloads : 1107


References :