Epr Study of Erythrocyte Properties After in Vitro Treatment With Urea and Hydrogen Peroxide

Anna Pieniazek; Krzysztof Gwozdzinski; Jan Czepas

Volume : III, Issue : IX, September - 2014

Epr Study of Erythrocyte Properties After in Vitro Treatment With Urea and Hydrogen Peroxide

Anna Pieniazek, Krzysztof Gwozdzinski, Jan Czepas

Abstract :

The aim of this study was to evaluate erythrocyte properties after in vitro treatment with urea and hydrogen peroxide. Changes in erythrocyte memane fluidity, conformational state of memane proteins and hemoglobin were investigated using EPR spectroscopy. The urea treatment resulted in increase of lipid memane fluidity in polar region and hydrophobic core indicated by 5– and 12–doxylstearic acids. The combination of urea and H2O2 lead to deeper changes in lipid microviscosity. The conformational state of memane proteins was measured using maleimide (MSL) and iodoacetamide (ISL) spin labels. ISL spectra showed a significant decrease in the memane proteins motion. However, in the combination of urea and H2O2 the spin labeled proteins mobility was similar to control value. Analysis of EPR spectra of MSL attached to hemoglobin showed significant increase in the motion of spin label residues after urea and H2O2 treatment. Similar results were observed for ISL but differences were insignificant.