Isolation and characterization of Chitinophaga sp. from paper mill polluted soil

Dhritiman Chanda; Sharma; G. D; D.K. Jha; M.Hijri; F. Al–Otaibi

Volume : III, Issue : XII, December - 2014

Isolation and characterization of Chitinophaga sp. from paper mill polluted soil

Dhritiman Chanda, Sharma, G. D, D. K. Jha, M. Hijri, F. Al Ndash Otaibi

Abstract :

Two Gram-negative, rod-shaped, deep yellow-pigmented bacteria were isolated from the polluted soil of paper mill contaminated with various heavy metals. The strains were tested for their resistance to different heavy metals (Ni,Cu, Zn and Cd) by their growth in nutrient oth tubes containing various concentrations of (0.1, 0.5, 2.0, 4.0 mM). The relative growths (%) at 2mM concentration were observed as (18.70 and 17.08 % ) in Ni, (13.71and 15.81%) in Cu, (18.63 and22.37%) in Zn and (18.36 and 8.39) in Cd. The heavy metal resistant in the two strains were found to be Zn>Ni>Cu> Cd at higher concentrations. The strains showed positive activity towards urease, nitrate, H2S production, citrate utilization, methyl red, Malonate utilization, oxidase production, starch amylase and showed negative activity against ONPG, phynylalanine, Lysine utilization and Voges Proskauer�s (VP) test and catalase activity. The strains were found susceptible to various antibiotics Vancomycin, Streptomycin, Amikacin, Ciprofloxacin. In silico study was conducted to understand the major evolutionary relationship among the different species of Chitinophaga species of nucleotide sequence of 16s ribosomal RNA with the isolated strains (KC602266 and KC602269) of Chitionophaga sp obtained from Gene Bank.