Volume : II, Issue : VII, July - 2013

Molecular Detection of Rotavirus Antigens in Stools of Children with Diarrhea in Khartoum State, Sudan

H. I. Hassan, A. Elnagi

Abstract :

Aims: To identify rotavirus antigens in stools of children with diarrhea using serological and molecular techniques in Khartoum State, Sudan. Materials and methods: Two hundred stool specimens were collected from 200 children less than 5 years of age suffering from diarrhea, all samples were tested for rotavirus antigens using latex agglutination technique and then 30 of them were diagnosed for rotavirus group A real time – PCR molecular technique. Results: Rotavirus antigens were detected in 42 subjects (21%) while 158 were found negative (79%). The study showed that, the disease incidence among males was (18%) and females were (26%). Most of the positive cases were among children less than one year of age (57.1%) followed by (38.1%) in children between 1–3 years of age, and lowest (4.8%) in the children less than 5 years. Using real time–PCR molecular technique rotavirus A were detected in 8 out of 20 positive latex agglutination samples (40%), while 12 samples (60%) showed no viral load. Ten cases of latex agglutination negative samples were confirmed by real time PCR, 5 of them were shown positive (50%) while the rest samples were confirmed negative. Conclusion: the study concluded that these differences in results were confirmed the importance of the application of rapid molecular methods for clinical services in hospital laboratories to provide definitive diagnoses.

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Article: Download PDF   DOI : 10.36106/ijsr  

Cite This Article:

H.I. Hassan, A.Elnagi Molecular Detection of Rotavirus Antigens in Stools of Children with Diarrhea in Khartoum State, Sudan International Journal of Scientific Research, Vol : 2, Issue : 7 July 2013

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